Tables

Table 1. - Relation of drugs and supplements ........................ 10

Table 2. - Distribution of the initial and effective sample according to age and sex of the subjects ....................... 13

Table 3. - Relation of the associated pathologies to the initial and effective sample ..................................................... 13

Table 4. - Relation of the family backgrounds relevant to the initial and effective sample ...................................... 13

Table 5. - Relation of incidents during the pregnancy ................. 14

Table 6. - Relation of incidents in the birth ...................... 14

Table 7. - Relation of the reactions to vaccines in the initial sample and the effective sample ............................... 15

Table 8. - Relation of behaviour regressions in the initial sample and the effective sample .......................................... 16

Table 9. - Description of the initial sample according to the arabinose, gluteomorphine and caseomorphine results obtained…………………………………………………. .... 17

Table 10. - Description of the effective sample according to the arabinose, gluteomorphine and caseomorphine results obtained…………………………………………………...... 18

Table 11. - Relation of the improvement level of the subjects from the effective sample after the drainage with homeospagyria ………………………………………........ 18

Table 12. - Relation of the improvement level of the subjects from de effective sample after the second phase ……............... 19

Table 13. - Evolution of the CARS punctuation in the effective sample in T1 and T2....................................................... 20

1 INTRODUCTION

In 1943 Leo Kanner, in the U.S., identified and described for the first time, the autism. Right after, in 1944, Hans Asperger, in Austria, described the diagram that has his same name. Some years after, from Lorna Wing’s contributions (1992), it’s incorporated the term Disturbance of the Austim Spectrum that in a more extended way includes the two told before and reflects the large variability that exists in the expression of this disorder.

In the last years the number of cases diagnosticated has considerably increased and, maybe because the diagnostic’s criteria has been enlarged and it could have been an influence, some authors admit that there is a continuous increase in the real number of cases. The autism it’s present in 4 men every 1 women. Nowadays it’s calculated that one every 170 born boys, suffers Disturbance of the Austim Spectrum (1).

Its characteristics are:

• Qualitative disturbance in the social interaction.
• Qualitative disturbance in communicating.
• Restrained standards in behaviour, interests and activities showing in many cases body stereotyped movements.

At present, even though the suspicion can appear sometimes at the age of 12 moths old, in the majority of cases the true diagnostic is done between the age of 30 and 36 moths old.

Framework reference

It is accepted that there are many factors that take part in the ethologic of this disorder, always over the base of a genetic component that, even though nowadays it’s hasn’t been possible to determine, it is thought that different chromosomes are involved. One of these factors could be a metabolic and intestinal dysfunction. Shattock (England, Sunderland University) and Reichelt (Norway) have formulated the hypothesis that the gluten’s peptides and the caseine exercise an ethological function in the pathogenic of the autism disorder. It is suggested that the psychological and behaviour alterations of the autism can be explained by the opioid activity peptides mentioned before, therefore these peptides pay attention to the opioids receptors of the brain, it affects the neurotransmission and provoke modifications on the behaviour. Excessive high levels of these opioids peptides have been measured excreted in the urine of the people with autism –Reichelt (1986) and Shattock (1990)–. Shattock and Whiteley have developed in the past years this theory (2). An enzymatic endopeptidases deficit, especially of Dipeptidil-peptidase IV (DPPIV), and it’s corresponding cofactors (vitamins and minerals), would explain why the digestion of some food (in this case the casein and gluten) could have been incomplete and would cause this abnormal high quantity of peptides in the digestive tract. These peptides, after being absorbed in the intestine, would pass the blood flow: part of it would be excreted by the kidneys and another part would cross the blood-brain barrier and arrive to the brain. Once there would become biologically active in the union with the opioids receptors and would produce interferences in the transition of the cerebral information.

An increase with the permeability intestinal would determine the anomalous absorption of an important quantity of these peptides, that because of its bigness, in physiological conditions of preserving the integrity in the intestinal wall, they wouldn’t have to be absorbed by the intestine. Wakefield and cols. (1998, 2000) studied a sample of 12 kids in a first work (3) and 60 kids in a second work (4), all of them diagnosticated with Disorder of the Autism Spectrum and determined by endoscope, that the 93% shower Hyperplasia Nodular Lymphoid. They concluded that this pathology could be considered as a subtle variation of the inflammatory sickness in the intestine. This intestinal alteration would explain by one side the anomalous absorption of the long-chain peptides and by other side the diminution of the vitamins and minerals absorption.

Dr.Shaw, director of the Great Plains Laboratory of Lenexa (USA), have founded very high levels of arabinose in the urine of many autism children. It suggests that this sugar would be a sub product of the Candides and those high levels in the urine would mean the existence of an intestinal Candida. This excessive growth of the yeast in the intestine would mean other interferences of the neurotransmission and it would contribute to worse the increase of the intestinal impermeabilization. It reports important improvements in the behaviours of children with autism, after doing an antifungal treatment. (5)

An expert group of the USA and Denmark have published in The Lancet magazine, a study where they report that a silence pandemic of the disorders in the neurological development exists caused by chemical toxic products sent to the environment. They have identified 202 industrial chemical products potentially harmful to the human brain that could cause, among others, autism, attention deficit, mental delay and brain paralysis. (6)

Dr. Waring described in 1993 that 90% of autism children had the enzymatic hepatic phenilsulfur-transferase levels decreased (7). This condition would determine a bigger difficult in removing out of the organism the sulphuric components by the hepatic metabolism.

According to this, and always from a special genetic configuration that hasn’t been determined yet and which it is believed that a variety of genes that interact with each other have an intervention in it, we could consider three factors present in the etiopathogeny of the autism:

• An enzymatic deficit that could cause a metabolic disorder
• An intestinal inflation accompanied by an increase of the intestinal permeability and dysfunction in the absorption of the nutrients.
• An accumulation of the toxins in the organism because of a malfunction in the elimination lines.

So, the people that have disorders of the Autism Spectrum would have to benefit from a treatment that would influence in these three aspects.

Objective

The objective of this study is to evaluate the efficiency of a biological and dietetic treatment, previously defined, to attenuate the autism’s symptoms, in a sample of this collective. And the purpose is double:

• On one side, give the families a treatment that can benefit their children and improve their quality of life.
• On the other side, stimulate the sanitary institutions in our country and go deeply on researching about the autism treatment, following this therapeutic orientation.

2 METHOD

2.5 Treatments and posologies

Table 1.- Relation between medicines and supplements

HOMEOSPAGYRIA + 6 years old – 1pill. 3/day

6-11years old – 4 drops 3/day

+ 12years old – 5drops 3/day

6-11years old- 4 drops 3/day +12years old- 5 drops3/day

Marbisan 3-5years old – 3drops 3/day 6-11years old – 4drops 3/day

+ 12years old – 5drops 3/day

DHA	Algatrium	50mg/kg/day
ENZIMS DIGESTIUS	Seren Aid	2 capsules 2/day(midday and
		night, before dinner)
Treatment Antiparasitic	Black Walnut	Following the treatment guides
Of Dr. Hulda Clark	Clove	of Dr.Clark according to age.
	Artemisia Absinthe
	Wormwood
	Ornitine
FLORA INTESTINAL	Symbiolact	1 sachet /day

(Rhamnosus)*
MULTIVITAMÍNIC	SuperNuthera	3 ml/morning
SUPLEMENT de Ca, Mg.	InmunoComplex	1 capsule/day
OLIGOELEMENTS	Ifigen; Si, Zn, Mg	5 ml./night
	Ifigen; Si, Zn, Mg, B,	5 ml./morning
	Mn, Cr
FORMULA MAGISTRAL	TMG 175 mg, Ac.	2 capsules/morning
	Folinic 200 mg, Vit.	2 capsules/night

B12 6 mg,

Tryptophan 150 mg

3 mg, Vit B3 5 mg, Vit B6 6 mg, Vit B12 6 mg.

HOMEOSPAGYRIA Lidospag 3-5years old – 3drops 2/day 6-11years old – 4 drops 2/day

+

12years old – 5drops 2/day

6-11years old – 4 drops 2/day

+

12years old – 5drops 2/day

6-11years old – 4 drops 2/day

+

12years old – 5drops 2/day

+

6 years old -1 pills 2/day

Sulkasar 3-5 years old – ½ pills 2/day

+ 6 years old -1 pills 2/day

HOMEOPATIA Serotoninum 2 granuls/nit Muriatium 5 CH

Dopamine 5 CH 2 granuls/matí

* It has only been administrated to one subject that showed high values of HPHPA (acid 3-(3hidroxifenil)-3 hidroxypropionic) in urine, indicate the presence of clostridia.

3 RESULTS

3.1 Description of the sample

3.1 Description of the sample

We’ve selected 20 subjects that had already made the first visit and the initial analyses.

The distribution by sexes it’s been: 16 men and 4 women.

Two of them are schooled in ordinary school with support, and 18 in school with special education. The subjects came from 9 different special education schools and 2 from ordinary schools.

From the second visit, we had 7 that left because of the following reasons:

• 4 of them because of family issues: familiar problems, difficulties following the diet and treatments,…
• 2 subjects experimented an increase in nervous and aggressively during the drainage.
• 1 subject after improving with the drainage and the antifungal treatment feels more nervous and unquiet when starting to take vitamins and other supplements.

Due to the interest that has for itself the entire datum concerning the set of the initial sample of the 20 subjects, it has been kept all the aspects of the clinical history the information regarding the 20 subjects and its called initial sample. The 13 subjects group that followed the treatment until the end it is called effective sample. So, from now on the initial sample will be the group of 20 subjects that made the first visit and whom we have results of the first analysis and the effective sample are the 13 subjects that followed the treatment until the end and whom we have also the second analysis and the second evaluation of the CARS test.

3.1.1 Distribution of the second sample according to sex and age

The distribution according to age it is collected in the next table.

Table 2.- Distribution of the initial sample and the effective according to the subject’s age

AGE (years)	3	4	5	6	7	8	9	10	11	12	13	T.
Initial sample	1	1	0	3	1	2	4	3	1	2	2	20
Effective sample	1	1	0	3	0	1	3	1	1	0	2	13

The distribution by sexes is i son the initial sample: 16 of masculine sex and 4 of feminine sex in the effective sample: 10 of masculine sex and 3 of the feminine sex.

3.1.2 Other associated pathologies

Table 3.- Relation of associated pathologies in the initial and effective sample

Pathology	Initial sample (cases)	Effective sample (cases)
Epilepsy subclinical	3	2
Down's Syndrome	1	1
Panhypopituitarism	1	1
Dysfunction of the suprarenal glandules	1	1
Malformation renal asymptomatic and betathalassemia	1	1

3.1.3 Family antecedents

In 7 of 20 cases we’ve considered the existence of familiar antecedents that could be relevant.

Table 4.- Relation of familiar antecedents of the initial an effective sample

Familiar antecedents	Initial sample (cases)	Effective sample (cases)
Fibromyalgia (*)	3	3
TEA (**)(Disorders of the Autism Spectrum)	3	2
Brothers with worse pathologies(***)	2	2

Parkinson: grandfather	1	1
Down's Syndrome: father's aunt	1	1
2 abortions before of the mother	1	1
Mother chronic vaginal Candidiasis	1	1

(*) In one case the aunt, in other case the grandmother and in other the aunt and the grandmother. (**) One case the mother’s cousin, one case the uncle and one case the grandfather. (***) One case of congenital cardiopathy and one case of death after 48h of being born.

3.1.4 Personal antecedents

PREGNANCY

We’ve registered the following incidents regarding the pregnancy that affected 10 cases of all 20.

Table 5.- Relation of incidents during the pregnancy

Incidence	Initial sample (cases)	Effective sample (cases)
FIV (fecundation in vitro)	1	0
Donors embryos	1	1
Very stressful fright	1	1
Stress, depression and hard flu + antibiotic therapy	1	1
Urinary infection + antibiotic therapy and vaginal pruritus during pregnancy	1	1
Pielonephritis + antibiotic therapy 8th month	1	0
Metrorrhagia and rest on the 1st, 2nd and 3rd trimesters	1	1
Albuminuria 6th month	1	1
Fainting with conscience lost at 8th month	1	1
Diabetes Mellitus in pregnancy	1	1

PART In 15 of 20 cases there’s been one of the following incidences

Table 6.- Relation of incidents during the delivery

Incidence	Initial sample cases	Effective sample cases
Caesarea	7	3
Distocic birth	5	4
Birth induction	4	3
Foetal suffering	2	1
Preterm at 7 months	1	1

Neonatal infection

1

REACTION TO THE VACCINES In 7 of 20 cases there was some kind of post vaccines reaction. The vaccines used were:

Table 7.- Relation of reactions to the vaccines in the initial and effective sample

Vaccine	Initial sample cases	Effective sample cases
Triple Viral	4	3
DTP Polio (diphtheria tetanus poliomyelitis)	2	1
Prevenar	1	1

The reactions that happened were:

• Edema of inferior limb (no local edema). Effective sample
• Fever + hypoglycaemia. Effective sample
• Adenophlegmon laterocervical with surgical drainage. Effective sample
• Edema local + fever + otitis. Effective sample
• Fever + vomit. Initial sample
• Regresion of development and increase of the autism symptoms. Initial sample.

REGRETIONS IN BEHAVIOUR

In 9 cases of 20 it was produced a regression on the behaviours in the development of the child, as it is collected by ages in the following table.

Table 8.- Relation of regressions of the behaviour in the initial and effective sample

Age	Initial sample (cases)	Effective sample (cases)
13 months	1	1
15 months	2	1
18 months	2	2
24 months	3	1
30 months	1	1

3.2 Results of the first urine analysis. Time 1

3.2.1 Initial sample

The results of the analysis of 20 subjects that started the study are represented in the table and following scheme.

Graphic 2.- Description of the initial sample according to the results obtained in arabinose, gluteomorphine and caseomorphine -20 subjects-

Arabinose

Table 9.- Description of the initial sample according to the results obtained in arabinose, gluteomorphine and caseomorphine.

Variable	Total	Gluteomorphine	Caseomorphine	Arabinose	All three
Gluteomorphine (gluten)	4	0	0	1	3
Caseomorphine (milk)	15	0	3	9	3
Arabinose	16	1	9	3	3

One subject scored negative in all three parameters. Three subjects scored positive in all three parameters.

3.2.2 Effective sample

The results of the 13 subjects that finished the study are present in the following scheme and table:

Graphic 3.- Description of the effective sample according to the results obtained in arabinose, gluteomorphine and case morphine -13 subjects-Time 1

Arabinose

Table 10.- Description of the effective sample according to the results obtained in arabinose, gluteomorphine and case morphine. Time 1

Variable	Total	Gluteomorphin e	Caseomorphin e	Arabinosa	All three
Gluteomorphine(glut en)